List Of Test And Sample Collection (Immunology)

All samples to be sent to Immunology Unit should use the specific containers according to the tests requested. Required volume, type of blood container and requirement of healthy control sample for each test are listed in the table below:

PROFILE		TEST		CONTAINER	VOLUME	SPECIMEN	REMARKS
Immunoglobulin Level		Immunoglobulin G		Plain tube	2 – 3.5 ml	Whole blood	Require healthy control sample at age matched
		Immunoglobulin A
		Immunoglobulin M
		Subclasses Immunoglobulin G1
		Subclasses Immunoglobulin G2
		Subclasses Immunoglobulin G3
		Subclasses Immunoglobulin G4
Standard Immunophenotyping Enumeration		T cell		EDTA tube	2 ml	Whole blood	Require healthy control sample (any age matched or adult sample accepted)
		B cell
		Natural killer cell
Phagocyte Function Test				Lithium Heparin without gel	4 ml	Whole blood	Require healthy control sample (any age matched or adult sample accepted)
PROFILE	TEST		CONTAINER		VOLUME	SPECIMEN	REMARKS
TB Spot Test			Lithium Heparin without gel		4 ml	Whole blood	Require healthy control sample (any age matched or adult sample accepted)
Allergy Test	Total Immunoglobulin E		Plain tube		2 – 3.5 ml	Whole blood	-
	Specific Immunoglobulin E House Dust Mite D. pteronyssinus D. farinae B. tropicalis Seafood Fish Crab Shrimp Pacific Squid Clam Food Allergen Egg white Egg yolk Milk Peanut Chicken Pet allergy Cat dander
Specific* Antibody Response	Pneumovax vaccination		Plain Tube		2 ml each for pre and post vaccination sample	Serum	Take the pre vaccination sample Vaccinate the patient Take the post vaccination sample 4 weeks after vaccination
	Tetanus toxoid vaccination

* For Specific Antibody Response, the tests should be requested via appointment. Appointment can be done by contacting 04-562 2231.

PROFILE		TEST	CONTAINER	VOLUME	SPECIMEN	REMARKS
Lymphocyte Proliferation test (LPT)			Lithium Heparin without gel	12 ml	Whole blood	Require healthy control sample (any age matched or adult sample accepted) Strictly sent to our lab within 8 hours after blood withdrawal. Failure to comply may cause the sample to be rejected
Extended Immunophenotyping	Switched memory B cells		EDTA tube	2 ml	Whole blood	Require healthy control sample (any age matched or adult sample accepted)
	CD40 Ligand Assay		Lithium Heparin without gel	2 ml	Whole blood	Require healthy control sample (any age matched or adult sample accepted) Strictly sent to our lab within 8 hours after blood withdrawal. Failure to comply may cause the sample to be rejected
	Bruton’s tyrosine kinase (Btk) function Assay		EDTA tube	2 ml	Whole blood	Require healthy control sample (any age matched or adult sample accepted)
Complement Assay	Complement 3 (C3)		Plain Tube	2 – 3.5 ml	Whole blood	-
	Complement 4 (C4)